RESEARCH AND DEVELOPMENT

RESEARCH AND DEVELOPMENT

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Research and Development


Science of the mysteries of life and pursuit of natural mechanisms.
 
Please refrain from reproducing all images and text on this site. 

Research and Development


Science of the mysteries of life and pursuit of natural mechanisms.
 
Please refrain from reproducing
all images and text on this site. 

 Research and Development of LEM & MAK

Recently, fermented foods have been attracting attention as health foods. Fermented foods [LEM][MAK] developed by Noda Shokubutsu Kogyo are packed with unknown power that has yet to be elucidated, with more than 90 patents, 100 papers published, and presentations at domestic academic conferences. We aim to establish a variety of backup systems, including product development by researchers in our own laboratory, sales and support by our sales staff, and the pursuit of evidence through our network of medical institutions and public organizations.


At Noda Shokukin, we place great importance on scientific evidence for our ingredients. We have obtained a number of various patents based on this data and strive to ensure the reliability and certainty of our products through academic papers.
>> frontiers

 Evidence: LEM Academic Reports list examples

There are dozens of Academic Reports of LEM, and the following list are some of them.

1.Kohayakawa S. et al. (2018) - Anti-allergic effects, New Food Industry 60, 42-48.
2.Kuroki T. et al. (2018) - Inhibition of influenza virus, Frontiers in Microbiology 9, 1164.
3.Xuan M. et al. (2017) - Cerebroprotective effects, New Food Industry 59, 1-14.
4.Horiuchi S. et al. (2016) - Neuroprotective effects, New Food Industry 58, 1-10.
5.Yamamoto Y. et al. (1997) - Immunopotentiating activity, Bioscience, Biotechnology, and Biochemistry 61, 1909-1912.
6.Sarkar S. et al. (1993) - Antiviral effect on herpes simplex virus, Antiviral Research 20, 293-303.
7.Morinaga H. et al. (1994) - Interleukin-1β mRNA expression, Japanese Journal of Cancer Research 85, 1298-1303.
8.Hibino Y. et al. (1994) - Interferon-γ and nitrite production, Immunopharmacology 28, 77-85.
9.Ichikawa Y. et al. (1991) - Intracellular calcium ion concentration, Journal of Traditional Medicines 8, 162-166.
10.Suzuki Y. et al. (1990) - Antitumor effect on colonic tumors, Journal of the Japan Society of Coloproctology 43, 178-191.
11.Shirahata A. et al. (1990) - Immunity improvement in HIV-infected patients, Immunopharmacology & Therapy 9, 103-106.
12.Suzuki H. et al. (1989) - Inhibition of HIV by lignin in LEM, Biochemical and Biophysical Research Communications 160, 367-373.
 

 ▒ Collaborative Research
We conduct joint research with universities and medical institutions and publish the results in academic papers. We are working to expand the possibilities of functional foods in collaboration with the Japanese Pharmaceutical Society, Complementary and Alternative Medicine (CAM), and the field of integrative medicine.
 
[Basic study]
■ UNIVERSITY OF TOYAMA
■ JOSAI UNIVERSITY
■ UNIVERSITY OF TSUKUBA
■ KINDAI UNIVERSITY
■ UNIVERSITY OF OCCUPATIONAL AND ENVIRONMENTAL HEALTH,JAPAN
■ Fukuoka University
■ Fukuyama University
 
[Clinical trials]
■ KEIJU MEDICAL CENTER
■ ORIENTM MITAKA-CLINIC
■ YUKAWA ICHO HOSPITAL
 

 Evidence: LEM Academic Reports example

There are many Academic Reports regarding several effect of LEM.
<Examples>

Treatment of HBeAg-positive chronic hepatitis with LEM (Author: Dr.Harada/Dr.Kanetaka)
Polysaccharides Improve Immunocompetence in HIV-Infected Patients (Dr.Shirahata etc.)
Treatment of hepatitis B patients with Lentinus edodes mycelium (Dr.Amagase)
 

Clinical Trial of Chronic Hepatitis B Treatment with LEM (Shiitake Mushroom Mycelia-G rowing Medium Extract)
 
<Product Summary>
   L EM is commercially available as a health food and has a high safety profile.
<Trial Summary> 
   A clinical study was conducted on 40 patients with chronic hepatitis B to evaluate the efficacy and safety of LEM. Patients were orally administered
  6g of LEM per day for 4 consecutive months.
<Test Results>
 ・Liver function was improved by LEM administration.
 ・Serum DNA polymerase activity and HBV-DNA levels were significantly decreased in HBV markers. 
 ・Seventeen of 40 patients tested negative for HBeAg and 10 patients seroconverted to HBeAg positive.
 ・In patients with chronic active hepatitis, the seroconversion rate was 36.8%. 
 ・The only side effects were mild abdominal distention and soft stools in one patient, suggesting that LEM is safe.
<Conclusion> 

  LEM may improve immunocompetence in HIV-infected patients, and further studies are expected.

① Key Points

The study aims to elucidate the antiviral & immunopotentiating effects of LEM against influenza virus.
Effects of LEM:
Direct inhibition of the early stages of viral infection (entry and uncoating).
Promotion of interferon-β (IFN-β) production, thereby activating immune responses.
Decreased viral load and dramatic reduction in lung inflammation.

② Results

In Vitro Experiments:
LEM inhibited plaque formation in a dose-dependent manner, achieving over 85% suppression at maximum concentration.
Minimal cytotoxicity was observed.
In Vivo Experiments:
Intranasal administration of LEM improved survival rates in mice.
Oral administration reduced the severity of pneumonia and decreased pulmonary lesions.
Early activation of IFN-β production effectively suppressed viral spread.
Overall Conclusion:
LEM has the potential to inhibit influenza infection through direct antiviral action and enhancement of immune responses.